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Can TempliPhi DNA “overload” the MegaBACE sample injection?

April 26, 2017dna injection megabace sample templiphi

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Can TempliPhi DNA “overload” the MegaBACE sample injection?

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Yes. Too much of any DNA can potentially overload MegaBACE capillaries. Sample-to-sample DNA concentration variability can potentially be reduced with TempliPhi by allowing reactions to go to completion (i.e. increase hours up to overnight) in comparison to amplifications that just went for a few hours. This ensures that all of the nucleotides in the reaction are incorporated into DNA. We recommend 150-350 ng of TempliPhi amplified DNA per sequencing reaction.