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Can Taq DNA Polymerase use RNA as a template, and generate false positives in “no-RT” controls?

April 26, 2017controls dna False polymerase positives RNA taq Template

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Can Taq DNA Polymerase use RNA as a template, and generate false positives in “no-RT” controls?

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Taq DNA Polymerase has an intrinsic RNA-dependent DNA polymerase activity (reverse transcriptase activity). However, this activity is very low and is only present under buffer conditions that are comp…