Can specimens be retrieved from the slide after analysis (or can ANY subsequent experiments be performed on the restriction fragments such as hybridization reactions)?
“Specimens” are prepared to generate High molecular weight DNA. HMW DNA is then loaded into the channels of a microfluidics Optical Chip and captured in parallel arrays in long, as single DNA molecules. Immobilized DNA molecules are retained electrostatically, interrogated with a restriction endonuclease, digested, and then stained. If the entire original specimen is used for sample preparation, there will be no specimen remaining for other testing. Because the HMW DNA is attached to the Optical Chip, and has been further processed, DNA retrieval and/or other testing would not be possible.
Related Questions
- Can specimens be retrieved from the slide after analysis (or can ANY subsequent experiments be performed on the restriction fragments such as hybridization reactions)?
- Can LOH (Loss of Heterozygosity) / CN (Copy Number) analysis be performed with GoldenGate genotyping?
- How is the chemical analysis of the urine performed?
