Can single-stranded DNA be prepared from the pTYB, pTXB, pTWIN and pCYB vectors?
Single stranded DNA can be prepared from these vectors. The antisense strand is rescued, so the Kunkel mutagenesis primers should be complementary and annealed to the antisense strand of the gene of interest. The CJ236 strain with M13K07 helper phage (NEB#N0315) has been tested. The IMPACT vectors contain a M13 origin, which should be compatible with other rescue systems such as CJ236 with VCSm13 helper. Other F’ hosts can be used to rescue single stranded DNA.
