Can PKC Inhibitors Block the Protection by Three-Cycle IP When They Are Administered Before the First Cycle of Transient Ischemia?
As shown in Figure 2, three cycles of IP significantly protected the heart from infarction: the median necrosis value was only 0.6% of the area at risk (necrosis values in all groups are expressed as the median) compared with 61.9% in the corresponding control group (P <.0001 versus control). One-cycle IP (with long stabilization) also protected the heart from infarction (36.7% of the area at risk infarcted, P = .05 versus corresponding control), but the extent of protection was significantly less than that obtained with three-cycle IP (P < .001 versus three-cycle IP). Shortening the stabilization period before inducing transient ischemia in hearts preconditioned with one-cycle IP from a total of 60 minutes to only 5 minutes significantly improved the protection obtained with one-cycle IP (10.4% necrosis in short-stabilization one-cycle IP versus 36.7% of the area at risk infarcted in the long-stabilization one-cycle IP, P < .05). Shortening of the stabilization period had no effect on
