Can other electroporation competent or chemically competent E. coli strains be used for transformation?
Yes, the CloneSmart ligations can be transformed into other strains of E. coli, but fewer clones will likely be obtained. Lucigen’s E. cloni electrocompetent cells yield over 20 billion clones per microgram of pUC19 DNA. Using less efficient cells will result in a proportionate decrease in the number of colonies obtained. To obtain the maximum number of transformants, use E.
