CAN ONE PREDICT THE RESISTANCE OF BULL SPERM TO CRYOPRESERVATION BY ANALYZING MOTILITY PARAMETERS BEFORE FREEZING?
L. Defoin, A. Granados and I. Donnay Abstract Batches of straws often need to be thrown away after freezing due to a too-few-number of motile or progressive sperm cells (spz), whereas the quality of the fresh sperm was considered as acceptable. Our objective was to evaluate whether variables related to velocity or linearity for fresh spz could help to predict the resistance to freezing and allow the discard of poor-quality batches before freezing. Motility traits of 20 ejaculates from 20 Belgian Blue bulls collected at an AI center were evaluated for motile spz both before and after freezing using Computer-Assisted Sperm Analysis (CASA, Spermvision; MinitÜb, Tiefenbach, Germany). Only six traits of motility showed a normal distribution in the population of motile spz and were kept for further analysis together with the proportion of motile (%mot) and progressive (%prog) spz: velocity on the curved line (VCL), velocity on the straight line (VSL), velocity on the average path (VAP), line
Related Questions
- If the quality (motility and number of sperm specimen) is inadequate after thawing, what steps should the patient/clinic take?
- Does anyone know of a relationship between excessive caffeine intake and sperm parameters - motility, morphology, etc?
- What is Embryo Freezing/ Sperm Freezing/ Cryopreservation?
