Can NUNC Brand CryoTube Vials be immersed in liquid nitrogen (liquid phase) for storage?
The CryoTube Vial of choice for liquid N2 immersion is the internally threaded design, incorporating a silicone gasket. At ultra low temperatures, any sealing material becomes inflexible. A theoretical risk of leakage is present when any mechanical closure is used. To facilitate leakproof storage, mechanically closed containers must be hermetically sealed in a protective envelope. The Nunc CryoSystem includes CryoFlex Tubing, a heat sealable polyethylene tubing designed for CryoTube Vial encasement. The use of CryoTube vials for freezing in liquid nitrogen (liquid phase) storage without CryoFlex Tubing poses a safety hazard. Warning: Do not use NUNC CryoTube Vials in liquid phase of liquid nitrogen unless correctly sealed in NUNC CryoFlex Tubing (Cat. No. 343958). Improper use may cause entrapment of liquefied nitrogen inside the vial and lead to pressure build-up, resulting in possible explosion or biohazard release. Use appropriate safety procedures as outlined in the Nalge Nunc Inte
