Can MycAssayTM kits be run on the Roche LightCycler 1.5?
The MycAssayTM PCR kits have been designed, optimised and validated to work on the market leading real-time PCR platforms (Cepheid SmartCycler, Roche LightCycler 2.0, ABI 7500 and Stratagene MX3000 series instruments).Some older PCR machines are not compatible with MycAssay™ products, such as the Roche LightCycler 1.5. The MycAssayTM kits will not work on the Roche LightCycler 1.5, because the LightCycler 1.5 is unable to specifically detect the HEX dye used on the Internal Amplification Control (IAC) beacon. The LightCycler 1.5 has three channels for detecting fluorescence: 530nm, 645nm, & 710nm. In contrast, the LightCycler 2.0 has six channels 530nm, 555nm, 610nm, 640nm, 670nm & 710nm. The MycAssayTM kits require the use of the 530nm channel to detect FAM dye for the target and the 555nm (referred to as 560nm in the IFU) channel to detect the HEX dye for the Internal Amplification Control (IAC). Therefore, due to limitations in the number of channels the MycAssayTM kit cannot be app
