Can my Nylon membranes be treated exactly like Nitrocellulose during colony hybridization?
A. One of our customers was wondering if drying E. coli on Nylon transfer membranes prior to denaturation effects the colony hybridization. Further, they were wondering if after drying the membranes, would treating with SDS before denaturation effect the hybridization? The answer is…NO, this will work! The hybridization will not be adversely affected. This is the procedure they used.
