Can mTFP1 be expressed in non-mammalian organisms?
mTFP1 has been successfully expressed in many different organisms, including E. coli and Arabidopsis thaliana. Because mTFP1, like other fluorescent proteins, does not require cofactors or special conditions to fold and develop fluorescence, it is expected that mTFP1 may be successfully expressed in almost any organism or cell type. However, researchers should keep in mind that the version of mTFP1 currently offered by Allele Biotechnology has been codon-optimized for efficient expression in mammalian cells (though high-level expression in standard lab strains of E. coli is possible without modification of the coding sequence).
