Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Can MBP fusions be purified in the presence of denaturants like urea or guanidine-HCl?

April 26, 2017denaturants fusions MBP presence purified urea

0

Posted

Can MBP fusions be purified in the presence of denaturants like urea or guanidine-HCl?

1 Answer

0

Posted

No, MBP’s affinity to amylose and maltose depends on hydrogen bonds, that in turn are positioned by the three-dimensional structure of the protein. Agents that interfere with hydrogen bonds or the structure of the protein interfere with binding as well.