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Can lysed whole blood BD Phosflow samples be stored for a longer time at -20°C – -80°C?

April 26, 2017BD blood longer lysed phosflow samples stored time

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Can lysed whole blood BD Phosflow samples be stored for a longer time at -20°C – -80°C?

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While fresh cells provide the greatest signal-to-noise ratio with BD Phosflow antibodies, we have heard from our collaborators that comparable results have been obtained by fixing cells, pelleting cells (per the recommended BD Phosflow protocols), then resuspending cells in PBS (without paraformaldehyde) and freezing the cell/PBS mixture at -80°C for storage. This method has only been tested for storage of several days. Please note, we have not extensively tested this method within BD Biosciences and the use of fresh lysed whole blood is recommended with BD™ Phosflow protocols.