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Can Leucoperm™ (BUF09) be used for staining intracellular markers by indirect flow cytometry?

April 26, 2017buf09 cytometry flow indirect intracellular leucoperm™ Markers staining Used

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Can Leucoperm™ (BUF09) be used for staining intracellular markers by indirect flow cytometry?

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Yes, but AbD Serotec always advises the use of a direct conjugate where available. The use of a secondary antibody may result in a higher level of background staining. If intracellular staining is preceded by the staining of a cell surface antigen then particular care must be taken when selecting a secondary antibody.