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Can Leucoperm™ (BUF09) be used for staining intracellular markers alongside cell surface staining?

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Can Leucoperm™ (BUF09) be used for staining intracellular markers alongside cell surface staining?

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Yes, but staining of the cell surface antigen (preferably with a directly-conjugated antibody) must be performed prior to fixation/permeabilization with Leucoperm™ and the subsequent intracellular staining.

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