Can jetPEI™ be used for transient as well as stable transfections?
Yes, jetPEI™ can be used for stable as well as transient transfections. To perform stable transfections, a supplementary protocol is available on our website. Start selection with the appropriate antibiotic 24 h to 48 h after transfection. In order to determine the correct amount of antibiotic for clone selection, we recommend performing a killing curve with your cell line using your culture conditions. We also recommend linearizing the expression plasmid prior to transfection in order to facilitate its integration into the genome.
