Can I view SYBR® Safe DNA gel stain with my ethidium bromide filter and camera settings?
Some ethidium bromide filters allow the transmission of all light above 500 nm. These filters (which are often yellow in color) and their associated camera settings can be used with SYBR® Safe DNA gel stain, usually with only minor adjustments to the exposure or gain. Other ethidium bromide filters (often red in color) only transmit light around or above 600 nm; these filters and their associated camera settings are not suitable for use with SYBR® Safe DNA gel stain. What other excitation wavelengths can I use to visualize SYBR® Safe DNA gel stain? SYBR® Safe DNA gel stain has two main excitation peaks: in the UV region at 280 nm, and in the visible region at 502 nm. Thus, 254 nm or 300 nm UV excitation will work, as will 488 nm lasers, 470 nm LEDs, and broad blue excitation (such as the Safe Imager™ blue-light transilluminator (Cat. no. S37102). Maximal excitation occurs at 502 nm; the Safe Imager™ blue-light transilluminator is therefore the best choice for excitation of SYBR® Safe D
