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Can I use yeast nitrogen base medium in place of yeast carbon base medium?

April 26, 2017base carbon medium nitrogen yeast

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Can I use yeast nitrogen base medium in place of yeast carbon base medium?

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10 Posted

No. Yeast nitrogen base (both with and without ammonium sulphate) contains nitrogen that can be used by cells instead of acetamide. To select for transformants, it is essential to plate the cells on YCB medium containing 5 mM acetamide (#B9017S).