Can I use water for RBC lysis?
For fresh blood: YES. Simply add 10 volumes of deionized water to the blood sample, vortex 20-30 seconds, centrifuge to pellet the cells, remove the red supernatant, and then proceed to extract DNA using AquaGenomic. For frozen blood: NO. Thawed RBC will not be lysed with water and you need to use our RBC Lysis Solution (Product # 4015), which is a modified hypotonic solution that lyses either fresh or frozen RBC.
