Can I use VIC, HEX or JOE on the SmartCycler®?
VIC, HEX, and JOE are very similar to TET and therefore may work with our calibration, but we cannot guarantee their performance since the base it is attached to may shift the optical spectrum. To use a dye that has not been calibrated at Cepheid, we suggest running as a simplex reaction and viewing the fluorescence reading in the adjacent channels to determine any cross-talk. If the desired result is not generated, the SmartCycler® and SmartCycler® II system may be recalibrated with the user-defined Optical Calibration feature in the SmartCycler®Software. Q: I created a FAM graph but it is displaying temperature. A: This is most likely due to the incorrect graph type. Check the graph definition in the Define Graphs screen to verify that the graph type is optics and not set to temperature. Q: I created a new graph but it is not listed in the View Results screen. A: When a new graph is defined in the Define Graphs screen it is saved and stored in the database but not associated with a r
