Can I use the “Warburg-Formula” with the BioPhotometer plus for determining the protein concentration at 280 nm?
No, this is unfornutately not possible, but in the result display the absorbance at 280 nm and 260 nm is shown. So it is possible to calculate the protein concetration via the “Warburg-Formula” manually: c(Protein) = 1,55 x E(280) – 0,757 x E(260). Please note that photometric measurement of the protein concentrion in the UV-range only suitable for pure and homogeneous protein solutions.
Related Questions
- For my DNA concentration determination (A260) the BioPhotometer shows a negative value at 230 nm and as a result no ratio A260/A230. What causes the negative A230 value?
- I cannot find the method key "Bradford" for protein determination in the BioPhotometer plus! Is this method still available in the BioPhotometer plus?
- Can I use the "Warburg-Formula" with the BioPhotometer plus for determining the protein concentration at 280 nm?
