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Can I use the validation system with other RNAi products like siRNA oligos?

April 26, 2017oligos products rnai siRNA system validation

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Can I use the validation system with other RNAi products like siRNA oligos?

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The pCMV-LUC(V) system is appropriate for screening siRNA, shRNA, or miRNA constructs. You must be certain to subclone into the validation vector the region of the cDNA targeted by your RNAi constructs; for example, if your siRNA targets the 5’ UTR, then you must clone into pCMV-LUC(V) the cDNA including 5’ UTR.