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Can I use the signal intensities of the Poly-A controls as standard curve for transcript abundance quantitation?

April 26, 2017Abundance controls curve intensities poly-a quantitation signal standard transcript

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Can I use the signal intensities of the Poly-A controls as standard curve for transcript abundance quantitation?

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These controls are only designed to provide a qualitative assessment on efficiency of the labeling assays, and NOT for the purpose of serving as a generic quantitative standard curve.