Can I use the same amount of any transfection reagent for different cell lines?
First, each of our transfection reagents is provided with an optimized protocol, and the amounts of reagent needed per well may be different between reagents. Please consult the product information that is provided with the transfection reagent for optimal use. Secondly, in most cases, the protocol that is provided with the product will provide you will an optimal range of transfection reagent to use per well. During product development, this range was determined to work well across a variety of cell lines. If you are still not achieving the performance you desire in your particular cell line, further optimization may be necessary. Please contact Technical Support for further assistance with optimizing your protocol.
