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Can I use the minilambda system with the galK system?

April 26, 2017galk system

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Can I use the minilambda system with the galK system?

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No. To use the galK system you need to transfer the BAC to one of the strains SW102, SW105, or SW106. The minilambda system, however, can be used to make conditional knock-out constructs without the need to remove the BAC from it’s original DH10B source.