Can I use the gWiz plasmids for stable transfection?
gWIZ plasmids are optimized for high-level transient expression studies. However, if you want to carry out stable transfection with gWIZ plasmids, the fastest way that it can be achieved is to do a co-transfection using gWIZ vector mixed with a second plasmid expressing a selection gene (e.g. neomycin resistance). In our studies, the optimal ratio of gWIZ to selection plasmid was 5:1. Twenty percent of neomycin resistant cell lines obtained this way also had the gene of interest stably integrated. In addition, a series of stable expression vectors were constructed using the gWiz backbone. Click here to see more information about the phCMV Mammalian Expression Vectors.
