Can I use the cDNA GEArrays for species other than human or mouse?
Based on our experience and that of our current customers, the mouse cDNA GEArrays can be used to analyze rat RNA given the high degree of identity between genes from the two different species. Some of our current customers have successfully used our human cDNA GEArrays to analyze RNA from a number of different species, such as pig, hamster, rabbit (Ning, XH, et al. (2002) J. Appl, Physiol. 92: 2200-2207.), monkey or other primate species (Bostik P, et al. (2004) J Virol. 78(3): 1464-72.). However, the probe synthesis and hybridization conditions must be optimized for your individual experiment. We recommend using our RT-Labeling Kit (L-01) instead of either of our other sample labeling kits. During that protocol, we also recommend using random primers (Promega Catalog Number C1181) and/or oligo-dT primers (Promega Catalog Number C1101) instead of our gene-specific primer mix, Buffer A. Start with final concentrations of 25 g/ml for the random hexamers and/or 50 g/ml for the oligo-dT p
