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Can I use SuperSignal West Pico Substrate with the identical protocol (i.e., same dilutions) I used with the GE Healthcare ECL Substrate or Pierce ECL Western Blotting Subtrate (Product # 32106)?

April 26, 2017dilutions GE identical Pico Protocol Substrate supersignal Used West

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Can I use SuperSignal West Pico Substrate with the identical protocol (i.e., same dilutions) I used with the GE Healthcare ECL Substrate or Pierce ECL Western Blotting Subtrate (Product # 32106)?

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No, SuperSignal Substrates require different dilutions and 5-minute incubation on the blot. You must use less (more dilute) primary and secondary antibodies with SuperSignal West Pico Substrate (see product instructions for recommended ranges). Please see Tech Tip # 21: Convert to SuperSignal West Pico Substrate from ECL Substrate.