Can I use Phusion Blood Direct PCR Kit for allele-specific PCR directly from blood (e.g. HLA-typing)?
It depends on the primers used. If the mismatch is located at the 3’ end and is not protected from cleavage by any modification, the answer is no. Phusion Blood DNA Polymerase has a strong 3’→5’ exonuclease activity which is able to cleave the mismatched base from the 3’end and continue amplification.
