Can I use OxyFLOW™ on adherent cells?
Yes. Please refer to the published article by Courter, Pereira and Baird entitled “Diesel exhaust influences carcinogenix PAH-induced genotoicity and gene expression in human breast epithelial cells in culture”, Mutation Research, 2007, 625:72-82. Question: I think I know which population of cells is showing DNA damage when they are treated with our compound. How can I specifically determine which cells are being affected? Answer: If there is an antibody that defines you cell population, then it is easy to use the multi-parameter acquisition ability of the flow cytometer to home in on these cells. When you use OxyFLOW™, you are only using 1 channel of the flow cytometer to measure the binding of the FITC-conjugated binding protein to 8-oxoguanine adducts produced as a result of oxidative DNA damage, regardless of how this damage has occurred. If you have an antibody or other expression marker that is, or can be, conjugated to a fluorochrome other than FITC, then after the primary incub
