Can I use other types of cell lysis and/or extraction buffer instead of the Extraction buffer provided in the Array Assay Kit?
Yes, it is possible to use other lysis buffers to lyse cells; however, the buffer must be free of Tris. The presence of Tris in cell lysates or extracted protein sample can adversely affect biotinylation of protein samples. For instance, the RIPA Lysis and Extraction buffer from Pierce Biotechnology contains Tris. If this buffer was used to extract proteins from cells, please be sure to remove the buffer from your protein extract and replace with the Labeling Buffer provided in the Antibody Array Assay Kit before proceeding to the next step. We recommend the following columns for buffer exchange (removing Tris): Spin columns included in the Antibody Array Assay Kit; Millipore, Microcon YM-10 filters (Catalog: 42406); Sephadex G-25 columns.
