Can I use other reagents instead of JFC for the histoprocessing?
Yes. The PATHOS is not a closed system for reagents. It was developed to allow the user to choose the proprietary JFC solution, or ethanol-isopropanol, or a combined use of ethanol-JFC, for use as follows: Ethanol-Isopropanol-Wax Ethanol-isopropanol protocol is recommended as the standard method for histoprocessing small to medium-large biopsies, provided that the fat content of the tissue blocks is kept to a minimum. It is a very cost-effective protocol for general histoprocessing. This is the recommended method for processing of renal biopsies. Ethanol-JFC-Wax Ethanol-JFC is for use with tissues where overnight fixation of tissue is uncertain, and for partially fixed tissues. In this instance, the ethanol acts as a post-fixative step, since JFC is not a fixative. This protocol is especially suited for routine use and for very fatty tissues, such as breast. The ethanol step will extend the reuse life of the JFC. JFC-Wax JFC solution is strongly recommended for tissues known to be well
