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Can I use organic solvents with SEC columns? What about surfactants like Triton or SDS?

April 26, 2017columns organic SDS sec solvents surfactants Triton

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Can I use organic solvents with SEC columns? What about surfactants like Triton or SDS?

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TSKgel SW/SWxl columns can tolerate up to 100% polar organics such as acetonitrile and methanol. TSKgel PW/PWxl columns can handle up to 20% polar organics in the mobile phase, and as high as 50% in special cases. Please contact the technical service group for these special cases. Using a surfactant in the mobile phase, e.g. 0.1% SDS will require that the column be dedicated to the application as the surfactant tends to irreversibly bind to the column and thereby alter the stationary phase.