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Can I use my TrueClone for in vitro transcription and translation?

April 26, 2017transcription Translation trueclone Vitro

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Can I use my TrueClone for in vitro transcription and translation?

1 Answer

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10 Posted

Yes, we have tested our TrueClones for IVTT in rabbit reticulocyte lysate systems and have seen protein production. However, if your clone is in the XL4 vector, it is necessary to liberate the insert and the sense T7 promoter from the vector.