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Can I use mTFP1 for fluorescence activated cactivated cell sorting (FACS)?

April 26, 2017Activated CELL FACs fluorescence mtfp1 Sorting

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Can I use mTFP1 for fluorescence activated cactivated cell sorting (FACS)?

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Yes, mTFP1 may be used for FACS, though a standard 488 nm laser excitation will not give optimal detection. For the best detection, one should ideally use a laser with a wavelength closer to mTFP1s excitation maximum of 462 nm.