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Can I use mRNA instead of total RNA as starting material?

April 26, 2017material mrna RNA total

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Can I use mRNA instead of total RNA as starting material?

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Purified poly(A) RNA has been successfully used as input to the comparable Ribo-SPIA-based RNA amplification systems. It will be necessary to use much lower amounts of input mRNA, comparable to mRNA present in 50-200 ng of a total RNA sample from the same tissue.