Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?

April 26, 2017FastLane lysate real-time rt-pcr

0

Posted

Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?

1 Answer

0

Posted

Increasing the volume of FastLane lysate will not necessarily increase PCR sensitivity and may, depending on the cell line used, reduce the linearity of the reaction.