Can I use Leinco Technologies antibodies for matched pair ELISA with a standard from another company?
Yes, but we recommend proceeding with caution. One observed problem with using antibodies from one company and a standard from a different company is there can be differences in the binding between antibody and recombinant protein. This could happen if there is a difference in the folding or sequence of the recombinant protein used as the standard vs. the recombinant protein used as the antigen for the antibody. Protein folding or sequence differences in the antibody-binding region can result in poor to no recognition of the standard.
