Can I use in LowCell# ChIP kit the sheared chromatin prepared with Shearing ChIP kit?
Yes, it is possible with small modifications: 30 µl of shearing buffer (from Shearing ChIP kit) is added per million cells. After shearing, transfer to a new tube the amount of chromatin needed (corresponding to a determined cell equivalent), and add Buffer B from the LowCell# ChIP kit to reach a total volume of 130 µl as shown below: • Add 30 µl of sheared chromatin to 100 µl of Buffer B (final: 1,000,000 cells /130 µl).
