Can I use Immobilon-PSQ for western applications involving small molecular weight proteins?
Millipore recommends Immobilon-P for westerns because of higher backgrounds observed when detecting proteins on Immobilon-PSQ. The smaller pore structure of Immobilon-PSQ also tends to limit the accessibility of bound proteins to antibodies. If optimization of Immobilon-P for detection of your protein is unsuccessful, Immobilon-P can be substituted. To minimize the background, remember that Immobilon-PSQ binds about three times as much protein as Immobilon-P. The amount of blocking solution used should be adjusted accordingly. Also, the length and number of washes may need to be increased to effectively wash excess antibody reagents from the pores.
