CAN I USE HYB & SEQ TO PERFORM RESEQUENCING IN BOTH FORWARD AND REVERSE DIRECTIONS?
You cannot re-sequence both strands of the same target DNA sequence on the same array simultaneously because then your single-strand DNA targets would hybridize to each other. However, you can use both strands individually with two different arrays (carrying the probes in appropriate orientation).
Related Questions
- WHAT IS THE TYPICAL ERROR RATE ASSOCIATED WITH USAGE OF HYB & SEQ RESEQUENCING? HOW DO REGIONS OF SECONDARY STRUCTURE AFFECT HYB & SEQ PERFORMANCE?
- CAN I USE HYB & SEQ TO PERFORM RESEQUENCING IN BOTH FORWARD AND REVERSE DIRECTIONS?
- DO I NEED TO PERFORM BACKGROUND SUBTRACTION AND NORMALIZATION FOR HYB & SEQ DATA?
