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Can I use GSEA to analyze a dataset that contains a single sample?

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Can I use GSEA to analyze a dataset that contains a single sample?

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Yes. However, GSEA has no way of ranking the genes in such a dataset. Therefore, you must rank the genes and then use GSEA to analyze the ranked list of genes. For more information, see the GSEA Preranked Page in the GSEA User Guide.

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