Can I use electorporation on the Whatman discs?
Yes – Whatman has documented that this does work. We have not tried it at the DGRC but we have received the following information from a colleague who succesfully used electroporation: After rinsing with the TE for a few seconds he added electrocompetent XLI Blue cells (the lab’s homemade) to the disc and incubated on ice for one minute. He then transferred the cells to a cuvette and electroporated. He plated the entire transformation on amp plates and obtained around 5 x 10^3 colonies.
