Can I use eFluorTM 605NC and eFluorTM 625NC conjugated antibodies instead of Pacific Orange?
The eFluorTM 605NC and eFluorTM 625NC conjugated antibodies can be used as an additional fluorochrome off the violet laser. Depending on your filter configuration of the cytometer, you may be able to use the same filter as Pacific Orange. Please refer to question above which indicates appropriate filters to use.