Can I use DMSO with CleanAmp(TM) Primers in my reaction?
A – DMSO improves PCR efficiency by disruption of GC-rich sequences. Typically, 0 to 10 percent DMSO is introduced into the reaction. DMSO can also inhibit PCR performance, so be sure that the percent DMSO introduced by CleanAmp(TM) Primers is not inhibitory to the reaction. At high DMSO percentages, the performance of CleanAmp(TM) Primers may be reduced. Therefore we recommend the addition of DMSO into your reaction only if absolutely necessary and to a maximum of 2 percent volume per volume.
