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Can I use chaotropes or detergents in my buffers when doing ion exchange chromatography?

April 26, 2017Buffers chromatography detergents doing exchange Ion

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Can I use chaotropes or detergents in my buffers when doing ion exchange chromatography?

1 Answer

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Yes. But be sure to use non-ionic detergents and chaotropes. Urea works well for ion exchange. Beware of guanidine salts as they are salts and will not allow your protein to bind.