Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Can I use an annealing temperature of 60ºC for QuantiTect Primer Assays so that I don’t have to change the cycling conditions I am currently using?

April 26, 2017annealing assays Conditions cycling don’t primer quantitect temperature

0

Posted

Can I use an annealing temperature of 60ºC for QuantiTect Primer Assays so that I don’t have to change the cycling conditions I am currently using?

1 Answer

0

Posted

QuantiTect Primer Assays are optimized for an annealing temperature of 55ºC. You can increase the annealing temperature to 60°C, but PCR performance (sensitivity and PCR efficiency) will definitely be reduced.