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CAN I USE ALTERNATIVE PROTOCOLS TO GENERATE SINGLE-STRAND DNA TARGETS?

April 26, 2017alternative dna protocols single-strand targets

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CAN I USE ALTERNATIVE PROTOCOLS TO GENERATE SINGLE-STRAND DNA TARGETS?

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An alternative approach is to perform PCR with two primers, one of which is tagged with the specific tag sequence and labeled with biotin. The resulting two strands can be separated by removing the biotin-tagged strand by binding to streptavidin beads and then eluting with 0.1 N NaOH.