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Can I transfer the cDNA from the panel to other types of PCR tubes that fit my thermal cycler?

April 26, 2017cDNA cycler fit panel PCR Thermal transfer tubes types

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Can I transfer the cDNA from the panel to other types of PCR tubes that fit my thermal cycler?

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Yes. If the 48-well panel does not fit your thermal cycler, the cDNA can be transferred to any appropriate type of PCR tubes. First, add 10 ul of dH2O to each well, incubate for 30 min. at room temperature, and mix gently to dissolve the cDNA. Second, transfer the cDNA from each well to a fresh tube, prepare and add 10 ul of 2X PCR premix for the first-round PCR.