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Can I substitute a different buffer and/or salt concentration in the column buffer?

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Can I substitute a different buffer and/or salt concentration in the column buffer?

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Yes, we have tried HEPES, MOPS, and phosphate buffers instead of Tris-Cl in the column buffer, at pH’s from 7.0 to 7.4 with identical results. NaCl or KCl concentrations of 25 mM to 1 M are also compatible with the affinity purification.

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